Data entry templates
| ID | Template | Project | Provider | Type | Category |
|---|---|---|---|---|---|
| COMET | COMET The comet assay, also called SCGE (Single Cell Gel Electrophoresis), is a rapid and informative method to detect DNA damage at single cell level used on many different cell types. The assay detects single and double strand DNA breaks as a consequence of a direct damage or as intermediate of DNA repair processes and it is successfully applied both in in vivo and in vitro genotoxicity testing. | RISKGONE | NILU | doseresponse | Genetic toxicity in vitro |
| CFE | Colony Forming Efficiency The colony forming efficiency assay (CFE) (also called clonogenic or plating efficiency assay) measures the ability of cells to survive and form colonies, which is an ultimate index of cytotoxicity. | RISKGONE | NILU | doseresponse | Cell Viability |
| ALAMARBLUE | Alamar Blue The Alamar Blue (AB) assay is a high throughput, cell metabolism-based method largely applied in toxicology and nanotoxicology to investigate cell viability (cytotoxicity), cell proliferation and cellular metabolic activity in response to chemicals and nanomaterials. | RISKGONE | NILU | doseresponse | Cell Viability |
| WST1 | WST-1 Assay for Cell Proliferation and Viability | GRACIOUS | BfR | doseresponse | Cell Viability |
| NRU | Neutral Red Uptake Assay | GRACIOUS | BfR | doseresponse | Cell Viability |
| HPRT | HPRT gene mutation assay - SWANSEA layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE | SWANSEA | doseresponse | Genetic toxicity in vitro |
| HPRTNILU | HPRT gene mutation assay - NILU layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE | NILU | doseresponse | Genetic toxicity in vitro |
| DEFAULT | In-vitro assay A generic dose response template. | IOM | doseresponse | Cell Viability | |
| DOSERESPONSE_TABLE | In-vitro assay (results as a table) A generic dose response template. | IOM | doseresponse | Cell Viability | |
| NEUTROPHIL_SURVIVAL | Neutrophil survival Neutrophil survival | POLYRISK/UU | doseresponse | Cell Viability | |
| DCFH | DCFH (acellular) | GRACIOUS | BfR | doseresponse | Oxidative Stress |
| CYTOKINES_RELEASE | Cytokines release Cytokines release | SABYNA | RIVM | doseresponse | Immunotoxicity |
| ENZYME | ACETYLCHOLINESTERASE - INHIBITION, ADSORPTION This type of NM characterisation is called biological characterisation based on enzyme inhibition/adsorption intensity. The more biologically reactive the suspended material (has a potential to inhibit enzyme) or the surface (has a potential to adsorb to the surface) the higher the inhibition of enzyme activity is. Results show that AChE is a promising candidate for ranking different NMs according to their adsorptive and inhibitory properties. | PLASTICFATE | University of Ljubljana | doseresponse | Oxidative Stress |
| MICRONUCLEUS | Long-Term 3D cytokinesis-blocked micronucleus (CBMN) assay The template for 3D cell models follows the structure of a 2D template. Still, it differs in the “Raw data” spreadsheet, where the metadata expected to be filled up follows the specificity of experiments to detect micronucleus in 3D spheroids (PATROLS, D4.1 SOPs for GIT and liver 3D advanced models optimised for long-term exposures. | PATROLS | SWANSEA | doseresponse | Genetic toxicity in vitro |
| CBMN | Acute 2D/3D cytokinesis-blocked micronucleus (CBMN) assay The micronucleus assay is considered the gold standard for detecting chromosomal damage in vitro. The assay is designed so that cells exposed to a genotoxic test agent result in chromosomal breakage forming small spherical nuclei (micronuclei) being detected as fixed DNA damage. | NANOINFORMATIX | FIOH | doseresponse | Genetic toxicity in vitro |
| BIOIMPEDANCE | Bio impedance - human cells Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE | University of Bergen (UiB) | doseresponse | Cell Viability |
| BIOIMPEDANCE_ECOTOX | Bio impedance - ecotoxicity Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE | University of Bergen (UiB) | doseresponse | Cell Viability |
| ECOTOX
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Ecotoxicity - fish, daphnia, algae, soil | NANOREG | JRC | ecotox | Short-term toxicity to aquatic inverterbrates |
| BIOACCUMULATION_MUSSELS
|
Bioaccumulation in mussels | SBD4NANO | INL | ecotox | Bioaccumulation: aquatic / sediment |
| OECD236_FET
|
OECD 236 Fish Embryo Acute Toxicity (FET) Test | SBD4NANO | INL | ecotox | Short-term toxicity to fish |
| ECOTOXTDRF | Ecotoxicity - dose response Dose response template for ecotoxicity experiment | IOM | doseresponse | Short-term toxicity to aquatic inverterbrates | |
| OECD202 | Ecotoxicity: Daphnia magna acute immobilisation test Short-term toxicity to aquatic inverterbrates OECD 202 | UoB | doseresponse | Short-term toxicity to aquatic inverterbrates | |
| OECD211 | Ecotoxicity: Daphnia magna reproduction test Long-term toxicity to aquatic inverterbrates OECD 211 | UoB | doseresponse | Long-term toxicity to aquatic inverterbrates | |
| PC_GRANULOMETRY_GRACIOUS
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Particle size distribution by ES-DMA, CLS (AUC) | GRACIOUS | JRC | pchem | Particle size distribution (Granulometry) |
| PC_DUSTINESS
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Dustiness with small drum | NANOREG | JRC | pchem | Dustiness |
| DENSITY_HEPYCNOMETER
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Density - He Pycnometer | GRACIOUS | JRC | pchem | Density |
| ZETAPOTENTIAL_RISKGONE
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Zeta Potential by ELS (RiskGone) The zeta potential is the property defining the surface charge of a material dispersed in a liquid. ELS technique is used to estimate the zeta potential of nanoparticles in suspension from their electrophoretic mobility. The zeta potential and the electrophoretic mobility are then related by the Henry equation. | RISKGONE | pchem | Zeta potential | |
| PC_SURFACECHEMISTRY_TGA
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Surface Chemistry (TGA) | GRACIOUS | JRC | pchem | Analytical Methods |
| PC_TGA_DEGRADATION
|
Degradation behavior by TGA | PLASTICFATE | BAM | pchem | Analytical Methods |
| PC_PY_GC_MS
|
Pyrolysis GC-MS for Qualitative Analysis of Polymer Materials Py-GC-MS | POLYRISK | VU | pchem | Analytical Methods |
| AIR_SAMPLING
|
Air sampling Air sampling | POLYRISK | UU | pchem | Analytical Methods |
| PC_MICROSCOPY_COUNTING
|
Particle counting by microscopy Microscopy measurements to estimate the number, size distribution and classification of particles | POLYRISK | Fraunhofer CSP | pchem | Analytical Methods |
| PC_VIBRATIONALSPECTROSCOPY_COUNTING
|
Particle counting by vibrational spectroscopy Vibrational spectroscopy measurements Such as µFT-IR or µRaman are used to estimate the number, size distribution classification and the type of micro and nanoparticles (in special plastics). The analysis of spectra and mapping images can be done using different techniques. | POLYRISK | Fraunhofer CSP | pchem | Analytical Methods |
| PC_SURFACECHEMISTRY_FTIR
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Surface Chemistry (FTIR) | GRACIOUS | JRC | pchem | Analytical Methods |
| PC_SPECTRA_RAMAN
(CC BY-SA 4.0) |
NANOREG Raman data template (updated by PLASTICFATE) | PLASTICFATE | CSIC | pchem | Analytical Methods |
| SSA_SEARSTITRATION
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Specific Surface Area by Sears Titration (for colloidal silica) | GRACIOUS | JRC | pchem | Specific surface area |
| HYDROPHOBICITY_CONTACTANGLE
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Hydrophobicity by Contact Angle | GRACIOUS | JRC | pchem | Water solubility |
| SURFACECHEMISTRY_XPS
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Surface Chemistry by X-ray Photoelectron Spectroscopy (XPS) | GRACIOUS | JRC | pchem | Surface chemistry |
| CRYSTALLINITY_XRD
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Crystallinity by X-Ray diffraction (XRD) | GRACIOUS | JRC | pchem | Crystalline phase |
| COMPOSITION_XRF
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Elemental composition and chemical purity by X-ray Fluorescence (XRF) | GRACIOUS | JRC | pchem | Analytical Methods |
| COMPOSITION_ICPMS
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Elemental composition by ICP-MS | GRACIOUS | JRC | pchem | Analytical Methods |
| COMPOSITION
(CC BY 4.0) https://doi.org/10.5281/zenodo.7751340 |
Define material composition Detailed information of material component (chemistry, role, identifiers). | HARMLESS | IDEA | pchem | Material composition |
| OMICS
(CC BY-NC-SA 4.0) |
Omics metadata А metadata map for omics data with columns relevant for nanosafety. Omics data stored in e.g. Gene Expression Omnibus (GEO), ArrayExpress, or PRIDE implement omics community standards, including minimum information requirements for omics experiments. However, the databases lack field-specific metadata, such as that defined within the nanosafety community, which leads to poor interoperability with other types of data and databases. | NANOREG2 | KI | metadata | Omics metadata |
| OMICSINVIVO
(CC BY-NC-SA 4.0) |
Omics in-vivo metadata OMICS template extended with fields for in-vivo studies | HARMLESS | KI,NRCWE | metadata | Omics metadata |
| PC_GRANULOMETRY_NANOREG
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Particle size distribution by TEM, DLS | NANOREG | JRC | pchem | Particle size distribution (Granulometry) |
| PC_GRANULOMETRY_DLS
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Hydrodynamic particle size by DLS | NANOREG | JRC | pchem | Particle size distribution (Granulometry) |
| PC_GRANULOMETRY_SAXS
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
SAXS | NANOREG | JRC | pchem | Particle size distribution (Granulometry) |
| PC_GRANULOMETRY_TEM
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Particle size distribution by TEM | NANOREG | JRC | pchem | Particle size distribution (Granulometry) |
| PC_GRANULOMETRY_NTA
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Particle size and particle counting by Nanoparticle Tracking Analysis NTA technique provides size characterization based on the free diffusion behavior of particles in solution. NTA measures particle diffusion by tracking the random motion of single particles in solution via high temporal-resolution video acquisition and enhanced contrast microscopy. The diffusion of the objects in suspension is measured with single-particle resolution, and hence it is particularly suitable for the characterization of size distribution for highly polydisperse nanoparticle populations. | NANOREG | JRC | pchem | Particle size distribution (Granulometry) |
| PC_GRANULOMETRY_NANOREG_RISKGONE
(CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 |
Particle size distribution by TEM, DLS,NTA (RiskGone) Multiple materials and results in JRC/NANoREG template style - modified by RiskGone | RISKGONE | pchem | Particle size distribution (Granulometry) | |
| DENSITY_EFFECTIVE
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Effective density Multiple materials and results in JRC/NANoREG template style | GRACIOUS | JRC | pchem | Density |
| DENSITY_EFFECTIVE_RISKGONE
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Effective density by VCM (RiskGone) The determination of the effective density of NM by the VCM (Volumetric Centrifugation Method) is based on the measurement of the volume of the pellet obtained by low speed, benchtop centrifugation of NM suspensions in a packed cell volume tube. | RISKGONE | pchem | Density | |
| ZETAPOTENTIAL
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Zeta Potential | GRACIOUS | JRC | pchem | Zeta potential |
| IL8_ELISA | [PATROLS template] ELISA Detection of IL-8 ELISA Detection of IL-8 | PATROLS | doseresponse | Immunotoxicity | |
| TNFA_ELISA | [PATROLS template] ELISA Detection of TNF-α ELISA Detection of TNF-α | PATROLS | doseresponse | Immunotoxicity | |
| TEER | [PATROLS template] TEER - Transepitelial Electrical Resistance TEER - Transepitelial Electrical Resistance | PATROLS | doseresponse | Cell Viability | |
| PATROLSAB | [PATROLS template] Alamar Blue Alamar Blue template by PATROLS | PATROLS | doseresponse | Cell Viability | |
| ECR
(CC BY 4.0) https://zenodo.org/record/4665253 |
ECR- Environmental Consumer Release The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. | GRACIOUS | LEITAT | exposure_release | Use and exposure information |
| ORE
(CC BY 4.0) https://zenodo.org/record/4665253 |
ORE- Occupational Release The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. | GRACIOUS | LEITAT | exposure_release | Use and exposure information |
| DISSOLUTION
(N/A) |
Dissolution, dynamic or static | GRACIOUS, GOV4NANO and NanoHarmony | pchem | Water solubility | |
| SSA_BET
(CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 |
Specific Surface Area by BET (Brunauer, Emmett and Teller) method | GRACIOUS | JRC | pchem | Specific surface area |
| NRF2ACTIVATION | NRF2 Activation assay This template allows data entry for multiple materials. Please specify the number of materials in the relevant web form field. | GRACIOUS | BfR | doseresponse | Cell Viability |
| CARBONYLATION | Determining Protein Carbonylation | GRACIOUS | BfR | doseresponse | Cell Viability |
| BARRIERCROSSING | Barrier crossing | NANOINFORMATIX | doseresponse | Barrier integrity | |
| REDOXPOTENTIAL | Redox Potential | NANOINFORMATIX | pchem | Oxidative Stress | |
| NOPRODUCTION | Nitric Oxide production | SBD4NANO | doseresponse | Oxidative Stress | |
| FRAS
(CC-BY 4.0) https://doi.org/10.5281/zenodo.7729589 |
Ferric Reduction Ability of Serum Ferric reducing ability of serum (FRAS) assay template. FRAS assay is an indirect assay, based on the principle of absorbance change due to reduction of an Fe3+ complex to Fe2+ complex, by the residual antioxidants in a sample, pre-incubated with a nanomaterial.The absorbance change is correlated to the antioxidant capacity of the serum: the more antioxidants species, the more intense the blue colour. Antioxidants may be suppressed by free radicals produced by nanomaterials, leading to oxidative damage, shown by a reduced blue colour in the assay. | HARMLESS | BASF | doseresponse | Oxidative Stress |
| HEMOLYSIS
(CC-BY 4.0) ? |
HEMOLYSIS ? | HARMLESS | BASF | doseresponse | Oxidative Stress |
| DISSOLUTIONIONS
(CC-BY 4.0) https://doi.org/10.5281/zenodo.7729600 |
Dynamic dissolution in lung stimulant fluid Dynamic dissolution of a test material in lung simulant fluids is monitored via a continuous flow system consisting of a heat cabinet with reservoir, flow-through cells, peristaltic pump set at 2mL/h and external autosampler. Leaching/biopersistence of relevant metals may be estimated after analysis of the samples in time by a suitable analytical method (i.e., ICP-MS) | HARMLESS | BASF | pchem | Water solubility |
| DISSOLUTIONAQUATIC
(CC-BY 4.0) https://doi.org/10.5281/zenodo.7729600 |
Dynamic dissolution (aquatic) Dynamic dissolution (aquatic) | HARMLESS | BASF | pchem | Water solubility |
| MODELS | Models metadata Models metadata | SBD4NANO | Maastricht University | metadata | Computational models metadata |
| CHARISMA_RR | Raman spectra metadata template Raman spectra metadata including links to spectra files | CHARISMA | pchem | Analytical Methods | |
| HTS_METADATA | Metadata including links to data files High Throughput Screening (HTS) metadata, with links to data files | HARMLESS | metadata | Cell Viability | |
| STIS | Short term intratracheal instillation STIS | HARMLESS | NRCWE | invivo | Acute and sub-chronic toxicity - pulmonary instillation |