Templates Wizard gallery
H2020 IMPTOX
| Template Wizard link | Template | Project/Provider | Type | Category | Status | |
|---|---|---|---|---|---|---|
| DEFAULT | In-vitro assay A generic dose response template. | /IOM | doseresponse | Cell Viability | published | |
| DOSERESPONSE_TABLE | In-vitro assay (results as a table) A generic dose response template. | /IOM | doseresponse | Cell Viability | draft | |
| NEUTROPHIL_SURVIVAL | Neutrophil survival Neutrophil survival | /POLYRISK/UU | doseresponse | Cell Viability | draft | |
| CYTOKINES_RELEASE | Cytokines release Cytokines release | SABYNA/RIVM | doseresponse | Immunotoxicity | draft | |
| ALAMARBLUE | Alamar Blue The Alamar Blue (AB) assay is a high throughput, cell metabolism-based method largely applied in toxicology and nanotoxicology to investigate cell viability (cytotoxicity), cell proliferation and cellular metabolic activity in response to chemicals and nanomaterials. | RISKGONE/NILU | doseresponse | Cell Viability | published | |
| BARRIERCROSSING | Barrier crossing | NANOINFORMATIX/ | doseresponse | Barrier integrity | published | |
| BIOACCUMULATION_MUSSELS | Bioaccumulation in mussels | SBD4NANO/INL | ecotox | Bioaccumulation: aquatic / sediment | published | |
| BIOIMPEDANCE | Bio impedance - human cells Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE/University of Bergen (UiB) | doseresponse | Cell Viability | published | |
| BIOIMPEDANCE_ECOTOX | Bio impedance - ecotoxicity Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE/University of Bergen (UiB) | doseresponse | Cell Viability | published | |
| CARBONYLATION | Determining Protein Carbonylation | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| CBMN | Acute 2D/3D cytokinesis-blocked micronucleus (CBMN) assay The micronucleus assay is considered the gold standard for detecting chromosomal damage in vitro. The assay is designed so that cells exposed to a genotoxic test agent result in chromosomal breakage forming small spherical nuclei (micronuclei) being detected as fixed DNA damage. | NANOINFORMATIX/FIOH | doseresponse | Genetic toxicity in vitro | published | |
| CFE | Colony Forming Efficiency The colony forming efficiency assay (CFE) (also called clonogenic or plating efficiency assay) measures the ability of cells to survive and form colonies, which is an ultimate index of cytotoxicity. | RISKGONE/NILU | doseresponse | Cell Viability | published | |
| CHARISMA_RR | Raman spectra metadata template Raman spectra metadata including links to spectra files | CHARISMA/ | pchem | Analytical Methods | draft | |
| COMET | COMET The comet assay, also called SCGE (Single Cell Gel Electrophoresis), is a rapid and informative method to detect DNA damage at single cell level used on many different cell types. The assay detects single and double strand DNA breaks as a consequence of a direct damage or as intermediate of DNA repair processes and it is successfully applied both in in vivo and in vitro genotoxicity testing. | RISKGONE/NILU | doseresponse | Genetic toxicity in vitro | published | |
| COMPOSITION | Define material composition (CC BY 4.0) Detailed information of material component (chemistry, role, identifiers). https://doi.org/10.5281/zenodo.7751340 | HARMLESS/IDEA | pchem | Material composition | published | |
| COMPOSITION_ICPMS | Elemental composition by ICP-MS (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| COMPOSITION_XRF | Elemental composition and chemical purity by X-ray Fluorescence (XRF) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| CRYSTALLINITY_XRD | Crystallinity by X-Ray diffraction (XRD) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Crystalline phase | published | |
| DCFH | DCFH (acellular) | GRACIOUS/BfR | doseresponse | Oxidative Stress | published | |
| DENSITY_EFFECTIVE | Effective density (CC BY-SA 4.0) Multiple materials and results in JRC/NANoREG template style http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Density | published | |
| DENSITY_EFFECTIVE_RISKGONE | Effective density by VCM (RiskGone) (CC BY-SA 4.0) The determination of the effective density of NM by the VCM (Volumetric Centrifugation Method) is based on the measurement of the volume of the pellet obtained by low speed, benchtop centrifugation of NM suspensions in a packed cell volume tube. http://dx.doi.org/10.2760/142959 | RISKGONE/ | pchem | Density | published | |
| DENSITY_HEPYCNOMETER | Density - He Pycnometer (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Density | published | |
| DISSOLUTION | Dissolution, dynamic or static (N/A) | GRACIOUS, GOV4NANO and NanoHarmony/ | pchem | Water solubility | published | |
| DISSOLUTIONIONS | Dynamic dissolution in lung stimulant fluid (CC-BY 4.0) Dynamic dissolution of a test material in lung simulant fluids is monitored via a continuous flow system consisting of a heat cabinet with reservoir, flow-through cells, peristaltic pump set at 2mL/h and external autosampler. Leaching/biopersistence of relevant metals may be estimated after analysis of the samples in time by a suitable analytical method (i.e., ICP-MS) https://doi.org/10.5281/zenodo.7729600 | HARMLESS/BASF | pchem | Water solubility | published | |
| ECOTOX | Ecotoxicity - fish, daphnia, algae, soil (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | ecotox | Short-term toxicity to aquatic inverterbrates | published | |
| ECOTOXTDRF | Ecotoxicity - dose response Dose response template for ecotoxicity experiment | /IOM | doseresponse | Short-term toxicity to aquatic inverterbrates | published | |
| ECR | ECR- Environmental Consumer Release (CC BY 4.0) The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. https://zenodo.org/record/4665253 | GRACIOUS/LEITAT | exposure_release | Use and exposure information | published | |
| ENZYME | ACETYLCHOLINESTERASE - INHIBITION, ADSORPTION This type of NM characterisation is called biological characterisation based on enzyme inhibition/adsorption intensity. The more biologically reactive the suspended material (has a potential to inhibit enzyme) or the surface (has a potential to adsorb to the surface) the higher the inhibition of enzyme activity is. Results show that AChE is a promising candidate for ranking different NMs according to their adsorptive and inhibitory properties. | PLASTICFATE/University of Ljubljana | doseresponse | Oxidative Stress | published | |
| FRAS | Ferric Reduction Ability of Serum (CC-BY 4.0) Ferric reducing ability of serum (FRAS) assay template. FRAS assay is an indirect assay, based on the principle of absorbance change due to reduction of an Fe3+ complex to Fe2+ complex, by the residual antioxidants in a sample, pre-incubated with a nanomaterial.The absorbance change is correlated to the antioxidant capacity of the serum: the more antioxidants species, the more intense the blue colour. Antioxidants may be suppressed by free radicals produced by nanomaterials, leading to oxidative damage, shown by a reduced blue colour in the assay. https://doi.org/10.5281/zenodo.7729589 | HARMLESS/BASF | doseresponse | Oxidative Stress | published | |
| HPRT | HPRT gene mutation assay - SWANSEA layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE/SWANSEA | doseresponse | Genetic toxicity in vitro | published | |
| HPRTNILU | HPRT gene mutation assay - NILU layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE/NILU | doseresponse | Genetic toxicity in vitro | published | |
| HYDROPHOBICITY_CONTACTANGLE | Hydrophobicity by Contact Angle (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Water solubility | published | |
| IL8_ELISA | [PATROLS template] ELISA Detection of IL-8 ELISA Detection of IL-8 | PATROLS/ | doseresponse | Immunotoxicity | draft | |
| MICRONUCLEUS | Long-Term 3D cytokinesis-blocked micronucleus (CBMN) assay The template for 3D cell models follows the structure of a 2D template. Still, it differs in the “Raw data” spreadsheet, where the metadata expected to be filled up follows the specificity of experiments to detect micronucleus in 3D spheroids (PATROLS, D4.1 SOPs for GIT and liver 3D advanced models optimised for long-term exposures. | PATROLS/SWANSEA | doseresponse | Genetic toxicity in vitro | published | |
| MODELS | Models metadata Models metadata | SBD4NANO/Maastricht University | metadata | Computational models metadata | published | |
| NOPRODUCTION | Nitric Oxide production | SBD4NANO/ | doseresponse | Oxidative Stress | published | |
| NRF2ACTIVATION | NRF2 Activation assay This template allows data entry for multiple materials. Please specify the number of materials in the relevant web form field. | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| NRU | Neutral Red Uptake Assay | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| OECD236_FET | OECD 236 Fish Embryo Acute Toxicity (FET) Test | SBD4NANO/INL | ecotox | Short-term toxicity to fish | published | |
| OMICS | Omics metadata (CC BY-NC-SA 4.0) А metadata map for omics data with columns relevant for nanosafety. Omics data stored in e.g. Gene Expression Omnibus (GEO), ArrayExpress, or PRIDE implement omics community standards, including minimum information requirements for omics experiments. However, the databases lack field-specific metadata, such as that defined within the nanosafety community, which leads to poor interoperability with other types of data and databases. | NANOREG2/KI | metadata | Omics metadata | published | |
| OMICSINVIVO | Omics in-vivo metadata (CC BY-NC-SA 4.0) OMICS template extended with fields for in-vivo studies | HARMLESS/KI,NRCWE | metadata | Omics metadata | published | |
| ORE | ORE- Occupational Release (CC BY 4.0) The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. https://zenodo.org/record/4665253 | GRACIOUS/LEITAT | exposure_release | Use and exposure information | published | |
| PATROLSAB | [PATROLS template] Alamar Blue Alamar Blue template by PATROLS | PATROLS/ | doseresponse | Cell Viability | draft | |
| PC_DUSTINESS | Dustiness with small drum (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Dustiness | published | |
| PC_GRANULOMETRY_DLS | Hydrodynamic particle size by DLS (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_GRACIOUS | Particle size distribution by ES-DMA, CLS (AUC) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_NANOREG | Particle size distribution by TEM, DLS (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_NANOREG_RISKGONE | Particle size distribution by TEM, DLS,NTA (RiskGone) (CC BY-SA 4.0) Multiple materials and results in JRC/NANoREG template style - modified by RiskGone http://dx.doi.org/10.2787/505397 | RISKGONE/ | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_NTA | Particle size and particle counting by Nanoparticle Tracking Analysis (CC BY-SA 4.0) NTA technique provides size characterization based on the free diffusion behavior of particles in solution. NTA measures particle diffusion by tracking the random motion of single particles in solution via high temporal-resolution video acquisition and enhanced contrast microscopy. The diffusion of the objects in suspension is measured with single-particle resolution, and hence it is particularly suitable for the characterization of size distribution for highly polydisperse nanoparticle populations. http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_SAXS | SAXS (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_TEM | Particle size distribution by TEM (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_SPECTRA_RAMAN | NANOREG Raman data template (updated by PLASTICFATE) (CC BY-SA 4.0) | PLASTICFATE/CSIC | pchem | Analytical Methods | published | |
| PC_SURFACECHEMISTRY_FTIR | Surface Chemistry (FTIR) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| PC_SURFACECHEMISTRY_TGA | Surface Chemistry (TGA) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| PC_TGA_DEGRADATION | Degradation behavior by TGA | PLASTICFATE/BAM | pchem | Analytical Methods | draft | |
| REDOXPOTENTIAL | Redox Potential | NANOINFORMATIX/ | pchem | Oxidative Stress | published | |
| SSA_BET | Specific Surface Area by BET (Brunauer, Emmett and Teller) method (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Specific surface area | published | |
| SSA_SEARSTITRATION | Specific Surface Area by Sears Titration (for colloidal silica) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Specific surface area | published | |
| SURFACECHEMISTRY_XPS | Surface Chemistry by X-ray Photoelectron Spectroscopy (XPS) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Surface chemistry | published | |
| TEER | [PATROLS template] TEER - Transepitelial Electrical Resistance TEER - Transepitelial Electrical Resistance | PATROLS/ | doseresponse | Cell Viability | draft | |
| TNFA_ELISA | [PATROLS template] ELISA Detection of TNF-α ELISA Detection of TNF-α | PATROLS/ | doseresponse | Immunotoxicity | draft | |
| WST1 | WST-1 Assay for Cell Proliferation and Viability | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| ZETAPOTENTIAL | Zeta Potential (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Zeta potential | published | |
| ZETAPOTENTIAL_RISKGONE | Zeta Potential by ELS (RiskGone) (CC BY-SA 4.0) The zeta potential is the property defining the surface charge of a material dispersed in a liquid. ELS technique is used to estimate the zeta potential of nanoparticles in suspension from their electrophoretic mobility. The zeta potential and the electrophoretic mobility are then related by the Henry equation. http://dx.doi.org/10.2760/142959 | RISKGONE/ | pchem | Zeta potential | published |