PATROLS - Physiologically Anchored Tools for Realistic nanOmateriaL hazard aSsessment
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 760813 PATROLS
patrols
CHEBI_46727 CHEBI_133326 CHEBI_51050 NPO_1542 NPO_1892 CHEBI_59999 ENM_9000006 NPO_1544nanoreg
NPO_1373 CHEBI_51050 NPO_1892 NPO_1542 NPO_354 ENM_9000006 CHEBI_133349 CHEBI_133326 CHEBI_59999 CHEBI_18246 NPO_606 NPO_157 NPO_943 CHEBI_3311 NPO_1550 CHEBI:18246 CHEBI_51135 CHEBI_133333 CHEBI_133337 CHEBI_133340 CHEBI_33418 NPO_401 ENM_9000007nanoreg2
CHEBI_51050 NPO_1373 NPO_1542 NPO_354 NPO_1892 CHEBI_133326 ENM_9000006 ENM_9000005 NPO_199nanotest
NPO_1548 CHEBI_51050 NPO_1373 NPO_1559enpra
CHEBI_51050 NPO_354 NPO_1542 NPO_1892 CHEBI_59999marina
CHEBI_51050 NPO_1373 NPO_354 NPO_1542 NPO_1892 CHEBI_16412 CHEBI_59999 CHEBI_133326nanogenotox
NPO_354 CHEBI_59999 CHEBI_51050 NPO_1373 NPO_1542patrols
Dextran Blue IL-8 ELISA IL-6 ELISA Human IL-6 DuoSet ELISA Human IL-8 DuoSet ELISA Human TNF-alpha DuoSet ELISA BCG Albumin Assay Urea Assay Kit Micronucleus Assay Cytokinesis-Block Proliferation Index TRYPAN BLUE Human IL-8/CXCL8 DuoSet ELISA Trypan Blue assay following 3D HepG2 spheroid repeated does ENM exposure Adenylate Kinase ToxiLight Assaynanoreg
MTS CELL VIABILITY ASSAY Impedance adherent cells ALAMAR BLUE Impedance flow cytometry NRU Spectrophotometric Method viability COMET RT-PCR MICRONUCLEUS ASSAY COMET FPG CBMN assay Manual scoring LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM ELISA Annexin V_PI (FACS) Griess Reagent LUMINEX® Reaction Oxygen Species Detection DLS Batch Dispersion Quality supplier WAXS VSSA SUPPLIER TGA Filtration ICP-MS DAPI Hoechst 33342 CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Ki-67 apoptosis cell cycle cleaved caspase-3 Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 Annexin V (Apoptosis) / PI (Necrosis) Nanoparticle tracking Analysis SEM SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261nanoreg2
COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Mercknanotest
radioactive tag labelling Expression of adhesion molecules flow cytometry DHE DCFH2-DA MTT Assay WST-1 LDH Propidium Iodide uptake IL6, TNF, etc By ELISA immunostaining COMET Cytokinesis Block Micronucleus Cytome (CBMN Cyt) NanoTest FeCell IC Chemical composition of particles HPLC-MS TEM SAD SAED DHE (Dihydroethidium) and Hoechst 33342 ICP-OES fluorescent rhodamine-labeledenpra
WST-1 LDH ALAMAR BLUE COMET Electron Paramagnetic Resonance (EPR) Fibroblast proliferationmarina
TEER NRU Resazurin LDH CFE Embryonic Stem Cell Test WST-1 ELISA Internalization of latex beads by cells, macrophage activity, inflammation Prothrombotic effects upon systemic administration Differential Centrifugal Sedimentation (DCS) SEM X-ray Photoelectron Spectroscopy (XPS) Electron Paramagnetic Resonance (EPR) TGA ALAMAR BLUE CFDA-AM OECD TG 202 OECD TG 225 OECD TG 217 Plant root length elongation Genotoxicity MICRONUCLEUS ASSAY ICP-MS ICP-OES DLS XRD Porosity OECD TG 210 OECD TG 201 systemic administrationnanogenotox
MICRONUCLEUS ASSAY COMET MLA-TK COMET FPG RICC TCC (Coulter counter) TRYPAN BLUE NRU XTT ICP-OES TEM SAXS TGA autopsy analysis blood kinetics analysis EDS DLS BET animal info Flow cytometry with PI staining XRDpatrols
A549 H441+dTHP-1 H441 A549+dTHP-1 NCI-H441 HepG2 PHHnanoreg
CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDMnanoreg2
CACO-2 A549 CALU-3 Caki-1 Hep3B NRK-52e carp leukocyte cell linenanotest
Lymphocytes leukocytes BALB/3T3 16HBE14o- A549 EC219 HCEC MDCK LLC-PK BeWo b30 Hepatocytes LN229 N11 ECp23 Kupffer cells K562 TK6enpra
LA-4 MH-S A549 HK-2 HepG2 mouse primary lung fibroblasts 16HBE human umbilical vein endothelial cells (huvec) 16HBE14o- J774 mouse primary lung fibroblasts, 3T3 and HFL-1marina
CACO-2 MH-S RAW 264.7 CALU-3 HMDM NIH3T3 mES TM3 Leydig cells ESnanogenotox
CACO-2 BEAS-2B human lymphocytes A549 16HBE L5178Y LH NHEK 16HBE14o- EFT