Development and implementation of Grouping and Safe-by-Design approaches within regulatory frameworks
https://cordis.europa.eu/project/id/646221
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 646221 NanoReg 2
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NPO_1373 CHEBI_51050 NPO_1892 NPO_1542 NPO_354 ENM_9000006 CHEBI_133349 CHEBI_133326 CHEBI_59999 CHEBI_18246 NPO_606 NPO_157 NPO_943 CHEBI_3311 NPO_1550 CHEBI:18246 CHEBI_51135 CHEBI_133333 CHEBI_133337 CHEBI_133340 CHEBI_33418 NPO_401 ENM_9000007nanotest
NPO_1548 CHEBI_51050 NPO_1373 NPO_1559enpra
CHEBI_51050 NPO_354 NPO_1542 NPO_1892 CHEBI_59999marina
CHEBI_51050 NPO_1373 NPO_354 NPO_1542 NPO_1892 CHEBI_16412 CHEBI_59999 CHEBI_133326nanogenotox
NPO_354 CHEBI_59999 CHEBI_51050 NPO_1373 NPO_1542nanoreg2
CHEBI_51050 NPO_1373 NPO_1542 NPO_354 NPO_1892 CHEBI_133326 ENM_9000006 ENM_9000005 NPO_199omics
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MTS CELL VIABILITY ASSAY Impedance adherent cells ALAMAR BLUE Impedance flow cytometry NRU Spectrophotometric Method viability COMET RT-PCR MICRONUCLEUS ASSAY COMET FPG CBMN assay Manual scoring LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM ELISA Annexin V_PI (FACS) Griess Reagent LUMINEX® Reaction Oxygen Species Detection DLS Batch Dispersion Quality supplier WAXS VSSA SUPPLIER TGA Filtration ICP-MS DAPI Hoechst 33342 CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Ki-67 apoptosis cell cycle cleaved caspase-3 Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 Annexin V (Apoptosis) / PI (Necrosis) Nanoparticle tracking Analysis SEM SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261nanotest
radioactive tag labelling Expression of adhesion molecules flow cytometry DHE DCFH2-DA MTT Assay WST-1 LDH Propidium Iodide uptake IL6, TNF, etc By ELISA immunostaining COMET Cytokinesis Block Micronucleus Cytome (CBMN Cyt) NanoTest FeCell IC Chemical composition of particles HPLC-MS TEM SAD SAED DHE (Dihydroethidium) and Hoechst 33342 ICP-OES fluorescent rhodamine-labeledenpra
WST-1 LDH ALAMAR BLUE COMET Electron Paramagnetic Resonance (EPR) Fibroblast proliferationmarina
TEER NRU Resazurin LDH CFE Embryonic Stem Cell Test WST-1 ELISA Internalization of latex beads by cells, macrophage activity, inflammation Prothrombotic effects upon systemic administration Differential Centrifugal Sedimentation (DCS) SEM X-ray Photoelectron Spectroscopy (XPS) Electron Paramagnetic Resonance (EPR) TGA ALAMAR BLUE CFDA-AM OECD TG 202 OECD TG 225 OECD TG 217 Plant root length elongation Genotoxicity MICRONUCLEUS ASSAY ICP-MS ICP-OES DLS XRD Porosity OECD TG 210 OECD TG 201 systemic administrationnanogenotox
MICRONUCLEUS ASSAY COMET MLA-TK COMET FPG RICC TCC (Coulter counter) TRYPAN BLUE NRU XTT ICP-OES TEM SAXS TGA autopsy analysis blood kinetics analysis EDS DLS BET animal info Flow cytometry with PI staining XRDnanoreg2
COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Merckomics
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CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDMnanotest
Lymphocytes leukocytes BALB/3T3 16HBE14o- A549 EC219 HCEC MDCK LLC-PK BeWo b30 Hepatocytes LN229 N11 ECp23 Kupffer cells K562 TK6enpra
LA-4 MH-S A549 HK-2 HepG2 mouse primary lung fibroblasts 16HBE human umbilical vein endothelial cells (huvec) 16HBE14o- J774 mouse primary lung fibroblasts, 3T3 and HFL-1marina
CACO-2 MH-S RAW 264.7 CALU-3 HMDM NIH3T3 mES TM3 Leydig cells ESnanogenotox
CACO-2 BEAS-2B human lymphocytes A549 16HBE L5178Y LH NHEK 16HBE14o- EFTnanoreg2
CACO-2 A549 CALU-3 Caki-1 Hep3B NRK-52e carp leukocyte cell lineomics