Advanced High Aspect Ratio and Multicomponent materials: towards comprehensive intelLigent tEsting and Safe by design Strategies
https://www.harmless-project.eu/
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 953183 HARMLESS
harmless
ENM_8000245 ENM_8000305 CHEBI_48730 NPO_1373 C569492 CHEBI_133340 NPO_729 CHEBI_133333 ENM_9000007 CHEBI_133337 NPO_354 CHEBI_51050 CHEBI_140503 CHEBI_82297 CHEBI_133354 NPO_1544 NPO_1548 ENM_9000254 CHEBI_46727 CHEBI_59999gracious
CHEBI_59999 NPO_1373 CHEBI_133326 NPO_1542 NPO_1544 ENM_9000073 NPO_1550 ENM_9000006 CHEBI_82297 CHEBI_51050 NPO_1892 NPO_707 NPO_589 CHEBI_46727 CHEBI_133349 NPO_1375 NPO_354 NPO_126patrols
CHEBI_46727 CHEBI_133326 CHEBI_51050 NPO_1542 NPO_1892 CHEBI_59999 ENM_9000006 NPO_1544nanoreg
NPO_1373 CHEBI_51050 NPO_1892 NPO_1542 NPO_354 ENM_9000006 CHEBI_133349 CHEBI_133326 CHEBI_59999 CHEBI_18246 NPO_606 NPO_157 NPO_943 CHEBI_3311 NPO_1550 CHEBI:18246 CHEBI_51135 CHEBI_133333 CHEBI_133337 CHEBI_133340 CHEBI_33418 NPO_401 ENM_9000007nanoreg2
CHEBI_51050 NPO_1373 NPO_1542 NPO_354 NPO_1892 CHEBI_133326 ENM_9000006 ENM_9000005 NPO_199calibrate
NPO_1373 NPO_1544 CHEBI_59999 CHEBI_51050 NPO_1892 CHEBI_82297cananolab
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CHEBI_51050 NPO_1892 ENM_9000241 NPO_606 NPO_1373 CHEBI_46666 CHEBI_82297omics
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WST-1 LDH assay ALAMAR BLUE CBMN CARBONYLATION BARRIERCROSSING CYTOKINES_RELEASE CFS FRAS DCFH2-DA ICP-OES SEM BET XRF HEMOLYSIS X-ray Photoelectron Spectroscopy (XPS) ATR-FTIR TEM AFM ICP-MS ES-DMA Sears Titrationgracious
WST-1 NRU NRF2ACTIVATION DCFH2-DA ROS/EPR with Tempone-h Electron Paramagnetic Resonance (EPR) FRAS sequential GIT Continuous Flow System (CFS) Dispersion stability Dynamic dissolution static solubility Electrophoretic Light Scattering X-ray Photoelectron Spectroscopy (XPS) XRF TEM BET XRD - crystalline phase He pycnometry TGA-MS Water contact angle GLU LDH TNFα MPPD v3.04 ICP-MS dustiness-small drum ICP-OES Dynamic dissolution LLF Dynamic dissolution PLF DLS AUC ES-DMA Sears titration inflammasome activation Volumetric Cell Measurement (VCM)patrols
Dextran Blue IL-8 ELISA IL-6 ELISA Human IL-6 DuoSet ELISA Human IL-8 DuoSet ELISA Human TNF-alpha DuoSet ELISA BCG Albumin Assay Urea Assay Kit Micronucleus Assay Cytokinesis-Block Proliferation Index TRYPAN BLUE Human IL-8/CXCL8 DuoSet ELISA Trypan Blue assay following 3D HepG2 spheroid repeated does ENM exposure Adenylate Kinase ToxiLight Assaynanoreg
MTS CELL VIABILITY ASSAY Impedance adherent cells ALAMAR BLUE Impedance flow cytometry NRU Spectrophotometric Method viability COMET RT-PCR MICRONUCLEUS ASSAY COMET FPG CBMN assay Manual scoring LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM ELISA Annexin V_PI (FACS) Griess Reagent LUMINEX® Reaction Oxygen Species Detection DLS Batch Dispersion Quality supplier WAXS VSSA SUPPLIER TGA Filtration ICP-MS DAPI Hoechst 33342 CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Ki-67 apoptosis cell cycle cleaved caspase-3 Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 Annexin V (Apoptosis) / PI (Necrosis) Nanoparticle tracking Analysis SEM SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261nanoreg2
COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Merckcalibrate
CellTiter-Glo (CTG) Automated hematology analyzer Short term oral toxicity Wright-Giemsa modified staining & optical microscopy Plasma (Heparin) Jejunum BAL cell celularity Histopathology COMET MICRONUCLEUS ASSAY AO Fluorescence Hyperspectral image analysis Quantitative PCR EPI-200-SIT OCL-200-EIT TEM DLS Supplier Life-history zFET Acridine Orange (AO) n.a.cananolab
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MTT Assay ALAMAR BLUE Calcein-Ethidium LDH CFE Cytokinesis Block Micronucleus Cytome (CBMN Cyt) DAN fluorimetric assay ELISA COMET CTA DCFH2-DA GSH MDA Electron Paramagnetic Resonance (EPR) Haemolysis assay TEM/SEM DLS Z-Potential in HAM'S-F12 Z-Potential in Water electroacoustic analysis on naturally concentrated samples Raman Spectroscopy UV TEER X-ray Photoelectron Spectroscopy (XPS) Infrared spectroscopy BET Z-Potential in DMEMomics
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A549 PMA -differentiated macrophages 'PMA-differentiated THP-1 cells' RTgill-W1 A549/ATCC PMA-differentiated THP-1 cells'gracious
HEK293patrols
A549 H441+dTHP-1 H441 A549+dTHP-1 NCI-H441 HepG2 PHHnanoreg
CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDMnanoreg2
CACO-2 A549 CALU-3 Caki-1 Hep3B NRK-52e carp leukocyte cell linecalibrate
BEAS-2B Platelets Red blood cells Hemoglobin White blood cells Granulocytes Lymphocytes Monocytes Polymorphonuclear basophils Polymorphonuclear eosinophils Polymorphonuclear neutrophils BAL cells BAL fluid cells Liver tissue Lung tissuecananolab
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A549 BALB/3T3 RAW 264.7 cell-free sheep erythrocytes CALU-3omics