AURORA - Developing an actionable European roadmap for early-life health risk assessment of micro- and nanoplastic (MNP) pollution
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under AURORA grant agreement No 964827.
nanoreg
NPO_1373 CHEBI_51050 NPO_1892 NPO_1542 NPO_354 ENM_9000006 CHEBI_133326 CHEBI_133349 CHEBI_59999 CHEBI_18246 NPO_606 NPO_157 NPO_943 CHEBI_3311 NPO_1550 CHEBI:18246 CHEBI_51135 CHEBI_133333 CHEBI_133337 CHEBI_133340 CHEBI_33418 NPO_401 ENM_9000007nanoreg2
CHEBI_51050 NPO_1373 NPO_1542 NPO_354 NPO_1892 CHEBI_133326 ENM_9000006 ENM_9000005 NPO_199nanoreg
MTS CELL VIABILITY ASSAY Impedance adherent cells ALAMAR BLUE Impedance flow cytometry NRU Spectrophotometric Method viability COMET RT-PCR MICRONUCLEUS ASSAY COMET FPG CBMN assay Manual scoring LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM ELISA Annexin V_PI (FACS) Griess Reagent LUMINEX® Reaction Oxygen Species Detection DLS Batch Dispersion Quality supplier WAXS VSSA SUPPLIER TGA Filtration ICP-MS DAPI Hoechst 33342 CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Ki-67 apoptosis cell cycle cleaved caspase-3 Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 Annexin V (Apoptosis) / PI (Necrosis) Nanoparticle tracking Analysis SEM SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 Alamar blue MTT assay ELISA MULTIPLEX Cytokine release method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 commercial kits manual count ATR-FTIR HAADF-STEM CHN-analysis ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX PrestoBlue protocol A13261 TRYPAN BLUEnanoreg2
COMET ALAMAR BLUE Impedance adherent cells CFE Dot-blot Nanoparticle Tracking Analysis DLS NTA Isothermal Titration Calorimetry (ITC) ZETA POTENTIAL CFDA-AM NRU OECD TG 202 OECD TG 201 EXPOSURE MTS CELL VIABILITY ASSAY Oxiblot® kit, Mercknanoreg
CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDMnanoreg2
CACO-2 A549 CALU-3 Caki-1 Hep3B NRK-52e carp leukocyte cell line